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Simple HPLC method for rapid determination of nicotine and cotinine in urine
Summary
Type:
HPLC
Application #:
105180
Detection:
UV, 260nm
Conditions:
eluent: dichloromethane - diisopropyl ether - methanol - 25% ammonia (62:30:7.9:0.1)
flow rate 2.0ml/min
Authors:
Horstmann M
Source:
J Chromatogr 344 (1985) 391 - 396
Details
Legend: Fig. 1. Chromatograms of extracts of A) smoker's urine, B) nonsmokers urine (blank urine, exceptionally not spiked with internal standard) and C) blank urine spiked with 1µg/ml nicotine and 1µg/ml c
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